Moradi, Hamideh (2015) Analysis of VEGF-1154G/A polymorphism and its relationship with recurrent implantation failure in infertile women referred to Royan Institute. Masters thesis, University of Zabol.
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Analysis of VEGF-1154GA polymorphism and its.pdf Download (246kB) | Preview |
Abstract
Most of the IVF-mediated embryos were failed due to unsuccessful implantation before clinical diagnosis. The process of successful implantation needs to develop an interaction between blastocyst and endometrial, in order to create blood vessels and angiogenesis. The Vascular Endothelial Growth Factor (VEGF) has important role in angiogenesis process. Many studies have shown the role of VEGF polymorphisms including: VEGF 1154 G/A in recurrent implantation failure. The VEGF 1154 G/A(rs1570360) polymorphism has different role in recurrent implantation failure in various geographical groups and races. In this study, healthy women with at least one successful pregnancy are considered as control group and women with recurrent implantation failure composed the case group, who after 10 or more embryo transplantation during 3 cycles of IVF didn’t become pregnant. Their genomic DNA was extracted from blood cells. Main primers were designed and PCR was done. The PCR product was sequenced by Sanger method. The analysis of SHESIS application showed that, there was no association between this SNP (at genotypic and allelic level) and the disease of interest. However, there was a hint of association based on the HWE P-values
| Item Type: | Thesis (Masters) |
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| Subjects: | Q Science > QH Natural history > QH426 Genetics R Medicine > R Medicine (General) |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 03 Apr 2016 07:02 |
| Last Modified: | 03 Apr 2016 07:02 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/181 |
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