Iranmanesh, S. (2017) The effect of Tricoderma on gene expression Carotenoid isomerase (CRTIOS) in Calendula officinalis under drought stress. Masters thesis, university of zabol.
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Abstract
Tracoderma are used as a biologyc fungicide, and also as a multiplier of plants growth and useful microorganism. Drough is the most important limitative factor in plant's growth and often reduse production..To investigate the effect of tricoderma fungi on expression of CRTIOS gene in ten genotype of (Calendula offcinalis L.) under drought stress, a factorial experiment based on randomized completely design with tree replications, have done in 2016 Zabol Agriculture Institute (chah-nime) and Agriculture Biotecnology Research Institute university of Zabol(Biocenter). The experiment treatments consist of two levels of Trichoderma fungi treatment of inoculation and non-inoculation(control), drought stress at three levels of irrigation(%50, %70 and %90 of field capacity) and ten genotypes(Isfahan teee genotypes.Tehran seven genotypes). The features which can be evaluated are: plant heighr, root length, fresh weight of the plant, dry weigh of the plant, root fresh weight, root dry weight, lateral branches, catalase enzymes, Guaiacol peroxidase, Ascorbate peroxidase, polyphenol oxidase, phenylalanine ammonia, chlorophyll a, chlorophy llb,total chlorophyll, carbohyhdrate and proline. The results showed that the main effect of tricoderma fungi, drought stress, genotype and their interactions on the most traits of the two plant in the probability level of one percent was significant. The results of drought stress and Trichoderma fungi on evaluating traits, showed that in all of the morphological traits, drought stress cause the reduced of morphological traits, photosynthetic pigments, increased antioxidant enzymes and proline. And Trichoderma also cause increasing all morphological traits, photosynthetic pigments, proline and some antioxidant enzymes. CRTIOS gene expression was performed with actin reference gene, gene expression was performed using Real Time PCR and data analysis with ΔΔCt method, then the expression of the gene for all treatments was considered. The results of study on the effect of Trichoderma fungi on three genotypes under drought stress in ten genotypes showed that the main effect of Trichoderma fungi treatments, drought stress, genotype and their interaction at (P≤0.01) level was significant. The highest expression of CRTIOS gene was observed in Isfahan (orange)(1.67) and the less expression of CRTIOS gene was observed in Tehran (36831)(0.53). The final resut showed that with inceasing the level of drought stress and use Tricodema we had the most expression of CRTIOS gene.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Tricoderma, Gene expression, Carotenoid isomerase, Phenolic compound |
| Subjects: | S Agriculture > SB Plant culture |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 19 May 2018 06:05 |
| Last Modified: | 05 Mar 2019 05:13 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/1798 |
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