Daerizadeh Minabi, S. (2016) Molecular Investigation Of Avicennia marina papulation in Hormozgan Province Using the Chloroplast Gene (tRNA- leu). Masters thesis, university of zabol.
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Abstract
The genetic structure of mangrove species is one of the best methods for management of mangrove forests. This method also evaluate our understanding regarding the evolutionary forces leading to biodiversity in this area. In this study, the chloroplast tRNA-Leu gene used for investigating the genetic structure and diversity of Avicennia marina. This gene is a reliable marker for genetic studies. In the present study DNA extraction from the frozen samples in liquid nitrogen and extraction buffer (CTAB 2%) had an acceptable quality and quantity for PCR. In this study, 600 sequences of 45 samples achived from the tRNA- Leu gene. 12 haplotypes obtained from three sampling stations also showed that the haplotype diversity of the three populations were moderate. All samples Avicennia marina samples from three stations in the two species showed their original Clyde. As it expected, Khamir and Qeshm samples showed one clade with 8 haplotypes and the other 4 haplotypes belonging to Jask area showed the another clade. The results showed that the hemozaygosity level of Qeshm and Khamir area were less than expected level which can cause increase in inbreeding coefficient between these species in the near future . Based on the genetic distance table, the lowest (0/5%) and highest (1.4%) rates of genetic distance were recorded between Qeshm and khamir respectively. Also the lowest (0/5%) and highest (1.9%) rates of genetic distance were recorded between Qeshm, khamir and Jask area respectively. According to the results of this study it seems there is an increase gene flow among populations of Khamir and Qeshm areas. Probably there have been less migration and gene flow between populations of Jask area and the two other populations (Khamir and Qeshm). The Fst Index and evolutionary trees also clearly showed a geographical expanding population between these areas According to the Fst level recorded between the samples, it seems that the genetic differentiation and gene flow from between Qeshm and Khamir area is more than the average with respect to Jask and the two mentioned regions .This genetic differentiation can be due to factors such as hydro-dynamic structure of the region (marine currents) in the Strait of Hormuz
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Mangrove, genetic differences, chloroplast gene, tRNA-Leu |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | Mrs najmeh khajeh |
| Date Deposited: | 19 May 2018 04:31 |
| Last Modified: | 03 Mar 2019 06:15 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/1770 |
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