Cloning of Hepatitis B Virus Surface Antigen (HBsAg) Encoded Gene Partial Sequence in E. coli

Shahreki Mojahed, M. (2014) Cloning of Hepatitis B Virus Surface Antigen (HBsAg) Encoded Gene Partial Sequence in E. coli. Masters thesis, University of Zabol.

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Hepatitis B Virus is one of the important causes of infectious disease with high rate prevalence of more than a million people per year, also it is the ninth factor that cause death around the world and the main cause of death in Iran. It has no specific treatment for the disease and the high prevalence of this disease requires prevention methods, particularly the development of immunization strategies. Therefore in this research, cloning of two native and synthetic fragment of virus surface antigen (HBsAg) based on genome sequences available in Gene Bank data bases was carried out. In first step, blood sample of patients with hepatitis B were collected from special diseased center of Zabol and after DNA extraction, cloning of the fragment by PCR was performed. Moreover virtual analysis such as modeling, isotope and epitope survey were done. The results of this study showed the expected fragment of 288 pb. Bioinformatic analysis showed the strong antigenicity performance of the synthetic fragment. The HBsAg fragment was cloned in the target vector. Overall, the result of this study provide a virtual data processing view of synthetic biology. In addition the recombinant synthetic construct could be introduced as a capable construct for development of recombinant vaccines

Item Type: Thesis (Masters)
Uncontrolled Keywords: Hepatitis B, Cloning, Epitope, Immunization, HBsAg, Virtual data processing, Synthetic biology
Subjects: Q Science > QH Natural history
Q Science > QR Microbiology
Depositing User: admin admin1 admin2
Date Deposited: 03 Apr 2016 05:53
Last Modified: 03 Apr 2016 06:00

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