Interaction of salinity, Bacillus subtilis and Glomus mosseae on cucumber damping off caused by Pythium aphanidermatum

Hosseyni, H. (2012) Interaction of salinity, Bacillus subtilis and Glomus mosseae on cucumber damping off caused by Pythium aphanidermatum. Masters thesis, University of zabol.

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Cucumber is one of the most important products in Iran that classified as semi-sensitive to salinity plants. In recent years due to changing weather conditions and limitations appearing in the quality of water resources and reduce, For the off- season and increased produce of cucumbers, more attention has been focused on the greenhouse cultures. Pests and pathogens are the most important factor limiting the cultivation of this product and lead to reduced yields in greenhouses. The biological control advantages compared to other methods to control the disease, the best way to control the disease in the greenhouse. In this study, the suspected infected plants to Pythium from commercial greenhouses growing cucumbers were collected, after isolation and morphological identification of fungal species Pythium, molecular identification was performed with specific primers for the dominant species. Then Antagonistic effects of Trichoderma virense and isolates of Bacillus subtilis strains on the fungus Pythium aphanidermatum in laboratory with cross-culture method was examined. Inhibitory effects of these isolates in the greenhouse with maycorrhizae fungus Glomus mosseae, and then the interaction of salinity with Glomus mosseae, isolates of the Bacillus subtilis BSP2 and B3E on damping off caused by pythium was evaluated. This study was done in a completely randomized design with four replications, Variance analysis of data was performed using SAS software and mean comparison were using LSD test at 5% level. Finally was found that disease severity in treatments with Trichoderma virense 31 percent, seed treatment with BSP2 and B3E isolates, respectively, 45 and 41%, the use of soil isolates and BSP2 and B3E respectively 46 and 42%. Also with colonization of seedling roots with maycorrhizae disease severity was 28 percent while the control plants infected with Pythium disease severity was 56%. By examining the interaction of salinity with Glomus mosseae, BSP2 and B3E, isolates of the species of Bacillus subtilis, were found that Bacterial isolates have little effect in reducing disease severity in salinity conditions, However, root colonization with the fungus Glomus mosseae in salinity levels 2, 4 and 8 dSm-1 were reduced disease severity significantly.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Pythium, Glomus mosseae, Bacillus subtilis, Trichoderma virense, Salinity
Subjects: S Agriculture > SB Plant culture
Depositing User: admin admin1 admin2
Date Deposited: 20 Sep 2017 08:07
Last Modified: 20 Sep 2017 08:07

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