Ultra Sound Assisted Extraction and Evaluation of Antioxidant Activity of L.amiaceas Using DPPH

Yosefi, Safiye (2014) Ultra Sound Assisted Extraction and Evaluation of Antioxidant Activity of L.amiaceas Using DPPH. Masters thesis, University of zabol.

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Abstract

The effects of free radicals and active oxygen species on biological molecules have created very problems in recent years. These compounds are known to be responsible for oxidative deterioration of vital molecules such as proteins, lipids and nucleic acids. Antioxidants protect biological systems against these factors and have been suggested to play a preventing role in human health. In the present study, the antioxidant activity of water and alcoholic extracts of L.amiaceas was determined. The extracts obtained by ultrasonic and traditional methods. 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) was added to the extracts and the antioxidant activity was determined by amount of reagent consumption. For tracking the progress of the reaction, high performance liquid chromatography (HPLC) was used. In order to optimize the extraction conditions, effective parameters such as extracting solvent, temperature and time were investigated. Finally, the results were analyzed by SPSS software. Analysis of variance (ANOVA) suggests that there are significant differences between extraction methods (P <0.05).

Item Type: Thesis (Masters)
Uncontrolled Keywords: Antioxidant activity,L.amiaceas, Ultrasonic assisted extraction, DPPH.
Subjects: Q Science > QD Chemistry
Depositing User: admin admin1 admin2
Date Deposited: 21 Jun 2017 07:47
Last Modified: 21 Jun 2017 07:47
URI: http://eprints.uoz.ac.ir/id/eprint/1588

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