Transient gene expression of GUS reporter gene in plants infected with Agrobacterium

Noory Sadeh, Gholam Ali (2015) Transient gene expression of GUS reporter gene in plants infected with Agrobacterium. Masters thesis, University of zabol.

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Gene transformation and production of transgenic plants has special importance. Development of suitable, fast, easy and efficient method to transfer genes into plants is one of the aims of genetic scientists. Gene transfer to plants performs to evaluate the transient and permanent gene expression. Transient expression is quick, easy and simple and is not influenced by position effect compare to the permanent expression. Overall, techniques for plant gene transformation could be divided into direct and indirect methods which biolistic and Agrobacterium-mediated gene transfer method are among the most applied methods. Due to the advantages of Agrobacterium-mediated gene transfer methods, including simple, inexpensive, accurate and being natural, in this study, development of a new method of Agrobacterium-mediated gene transfer to plants through the wooden vessel and transient expression of GUS reporter gene was investigated. Hence pCAMBIA1305.2 plasmid harbouring Intron-GUS reporter gene was transferred to the melon mildew Zabol (Cucumis melo), parsley (Petroselinum crispum) and pinto beans (Phaseolus vulgaris) using Agrobacterium. The results of PCR and Immunohistochemical GUS assay showed successful gene transfer to the plants. The established method requires simple facilities and easily performs, hence, it could be replaced with other methods of Agrobacterium-mediated gene transfer to plants.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Agrobacterium, GUS reporter gene, pCAMBIA1305.2 plasmid, Cucumis melo, Petroselinum crispum, Phaseolus vulgaris
Subjects: S Agriculture > SB Plant culture
Depositing User: admin admin1 admin2
Date Deposited: 21 Jun 2017 06:47
Last Modified: 21 Jun 2017 06:47

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