Transcriptomic Analysis of Gene Expressed Related to Cellulose Production in Some of Cellulose Decomposer Fungi

Leylai, S. (2011) Transcriptomic Analysis of Gene Expressed Related to Cellulose Production in Some of Cellulose Decomposer Fungi. Masters thesis, University of zabol.

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Cellulose is the most important polysaccharide in cell wall composition and is one of the most abundant hydrocarbons in nature. It constitutes 40 percent of plant vital mass. A complex of cellulase acts synergistically and break _-1, 4 linkages of cellulose chain. At least three cellulase enzymes named Betaglucosidase (EC, endoglucanase (EC and exoglucanase (EC are taken part in cellulose degradation .so, their structural features have also effects on cellulose degradation. Active fungal species in decomposition of cellulose and CMC environments represented by arsenate molybdate spectophotometry cellulase activities were evaluated. Four hight active species Trichoderma longibrachiatum, Trichoderma viride, Aspergillus terreus Penicillium oxalicum on substrate used for sequencing genes beta 1 and 4, endo glucanase were used. RNA isolation and DNA exteraction were performed by guanidine iso-tiusianat and CTAB metod, respectively. The amplified genes were sequenced and analyzed by gene sequencing then b ioinformatics analyze was done from their transcript amino acids. They showed high sequence homology about 97% to 98% with the same gene sequences as described in the NCBI database. For each isolate two domains were identified in databases. Molecular weight of the protein obtained,isoelectric points and areas for digestion enzyme were estimated in sequences studied. Based on the required data from BLAST for Nucleic acids and amino acids, these sequences were related to beta-1 , 4 enduglucanase or EG1. Analysis of domain and conserved sequencing and prosite were determined by sequencing the CDS related to same proteins which have high enzyme activity. The sequences was submitted to the NCBI database. Our results indicate that in our fungi species studied by hight cellulose activity there is an correlation between this high activity and gene expression so these species could be used in industry and fuel production biothecnology programs

Item Type: Thesis (Masters)
Uncontrolled Keywords: beta 1, 4 Endo glucanase, Trichoderma viride, Trichoderma longibrachiatum, Aspergillus terreus and Penicillium oxalicum, gene expression
Subjects: S Agriculture > SB Plant culture
Depositing User: admin admin1 admin2
Date Deposited: 21 Jun 2017 06:40
Last Modified: 21 Jun 2017 06:40

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