Assessment of genetic diversity of spring and winter rapeseed (Brassica Napus L.) Genotypes, using ISJ marker

Darvishnia, F. (2011) Assessment of genetic diversity of spring and winter rapeseed (Brassica Napus L.) Genotypes, using ISJ marker. Masters thesis, University of Zabol.

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Abstract

To investigate the genetic diversity among 21 rapeseed (Brassica Napus L.) cultivars, semi-random ISJ molecular marker was used. Two different sets of semi-random primers were used in which primers are designed based on intron - exon splice junction sequences. Ten semi-random primers produced a total number of 116 bands of which 107 (92%) bands were polymorphism. No significant differences were found between IT and ET primers with regard to polymorphic bands. ET15-35 and ISJ3 primers produced 16 and 5 polymorphic bands, respectively. Average numbers of polymorphic bands were 11/6 per primer. According to UPGMA cluster analysis and Dice similarity matrix, rapeseed cultivars were divided in 3 groups. Based on similarity matrix, KS- 11and Kiloz had the highest (0/86) and Licord, Option504 the lowest (0/37) similarity. Both Iranian and foreign cultivars were grouped together, indicating presumably they are of the same origin

Item Type: Thesis (Masters)
Uncontrolled Keywords: Primer, Genetic, Diversity, Rapeseed, Molecular Marker ISJ
Subjects: S Agriculture > SB Plant culture
Depositing User: admin admin1 admin2
Date Deposited: 28 Feb 2016 09:41
Last Modified: 28 Feb 2016 09:41
URI: http://eprints.uoz.ac.ir/id/eprint/151

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