Darvishnia, F. (2011) Assessment of genetic diversity of spring and winter rapeseed (Brassica Napus L.) Genotypes, using ISJ marker. Masters thesis, University of Zabol.
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Abstract
To investigate the genetic diversity among 21 rapeseed (Brassica Napus L.) cultivars, semi-random ISJ molecular marker was used. Two different sets of semi-random primers were used in which primers are designed based on intron - exon splice junction sequences. Ten semi-random primers produced a total number of 116 bands of which 107 (92%) bands were polymorphism. No significant differences were found between IT and ET primers with regard to polymorphic bands. ET15-35 and ISJ3 primers produced 16 and 5 polymorphic bands, respectively. Average numbers of polymorphic bands were 11/6 per primer. According to UPGMA cluster analysis and Dice similarity matrix, rapeseed cultivars were divided in 3 groups. Based on similarity matrix, KS- 11and Kiloz had the highest (0/86) and Licord, Option504 the lowest (0/37) similarity. Both Iranian and foreign cultivars were grouped together, indicating presumably they are of the same origin
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Primer, Genetic, Diversity, Rapeseed, Molecular Marker ISJ |
| Subjects: | S Agriculture > SB Plant culture |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 28 Feb 2016 09:41 |
| Last Modified: | 28 Feb 2016 09:41 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/151 |
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