Imani, E. (2014) The effect of Aflatoxin B1 in nuclear factor kappa b, NF-kb in human peripheral mononuclear cells in vitro condition. Masters thesis, university of zabol.
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The effect of Aflatoxin B1 in nuclear factor kappa b, NF-kb in human.pdf Download (93kB) | Preview |
Abstract
Nowadays, AFB۱ is one of the most bio-hazardous toxins particularly in agricultural products everywhere. In order to investigate the impact of AFB۱ on human immune system, an experiment was conduct on human PBMCs in vitro. Whole bloods from 2o volunteers were obtained and mixed. Using ficoll gradient procedure pure PBMCs were isolated and suitably seeded in 24 wells culture plate. PBMCs were exposed with different doses of AFB۱ (0 as control, 10 and 100 ng/ml). Real time quantitative (q) PCR assay was conducted using specific primers NF-kB and Beta-actin (as internal control). The results show AFB۱ significantly increased in both 10 and 100 ng/ml, but NF-kB up-regulation in 100 ng/ml were also statistically significant, compared to those in 10 ng/ml. Immunosuppressive effects of AFB۱ show the importance of choosing strategist to reduce and prevent AFB۱ toxicity...
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | AFB۱, NF-kB, Real time qPCR, PBMCs, upregulation |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 10 Jun 2017 06:29 |
| Last Modified: | 10 Jun 2017 08:10 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/1482 |
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