Study on genetic and pathogenic diversity of Ascochyta rabiei isolated from chickpea in Lorestan province

Rahimi, M. (2012) Study on genetic and pathogenic diversity of Ascochyta rabiei isolated from chickpea in Lorestan province. Masters thesis, university of zabol.

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Ascochyta blight caused by Ascochyta rabiei (Pass.) Lab. is one of the most devastating chickpea disease. To determine the genetic and pathogenic diversity of the pathogen, 53 isolates from Ascochyta rabiei were collected from eight regions in Lorestan province. The collected samples were cultured on Chikpea Seed Meal Dextrose Agar. For identification of pathogenic diversity, the isolates were divided into seven groups based on molecular methods and SSR markers. From each group one isolate as an indicator was randomly selected and pathogencity tests conducted using complated randomly design on seven differentioal chikpea cultivars in greenhouse conditions. Two weeks old seedlings inoculated by spraying spore suspension. After tree weeks diseased isolates were investigated for ascochyta blight using 1-9 scale and based on pattern of ICARDA pathogencity reaction, two pathotype were identified including pathtypeI from Chegeni, Nourabad, Khorramabad, Kuhdasht regions and pathotypeIII from Alashtar and Azna regions. Genetic diversity from seven sampling regions studied based on using A. rabiei specific microsattelite marker. Data in final analysis using SSR five pair selective primers and designing dendrogram tree, isolates with 74% coefficient similarity divided in to 16 groups. Results of precent study can be exploited in improvement of resistance cultivars and developments of quarantine proceedings.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Pathogencity, Microsattelite, Ascochyta rabiei, Genetic diversity
Subjects: S Agriculture > SB Plant culture
Depositing User: admin admin1 admin2
Date Deposited: 31 May 2017 05:31
Last Modified: 31 May 2017 05:31

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