Abuli, S. (2014) Study of polymorphism of FABP4 gene and its association with growth traits in Sistani and Dashtyari cattle. Masters thesis, university of zabol.
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Abstract
One of animal breeding advantages is recognition of genes which are beneficial for productive features and also are named candidate genes. FABP4 gene is obvious in adult fat cells and also is so important in biological processes which are related to lipids such as interface in metabolic paths and increasing fat tissues, and as a result strong will to compound with fatty long acids. In current study for existence of polymorphism in exon 3 of FABP4 gene and its relation to growth traits. Samples of 45 sistani and Dashtiari cows from sistan and baluchestan have been provided randomly from abdominal vein . DNA extracted from full blood and its quality investigated by electrophoresis of agarose 1% gel. A polymerase chain reaction was done by using a special primer pairs. Based on the patterns of size of bands, genotype of cattel was determined that it was resulted from endonuclease enzyme digestion NlaIII (RFLP) on the PCR products and electrophoresed on the agarose 2.8%. Genetic and population structures in FABP4 region was analyzed by POPGENE 3.2 software. The patterns of band led to the identification of three genotypes AA, AB and BB with frequency of 0.67, 0.3, and 0.03 in Sistani and with frequency of 0.73, 0.27 and 0 in Dashtiari, respectively. The frequency of allele A and B Alleles in exon 3 of FABP4 were 0.82 and 0.18 in Sistani and 0.87 and 0.13 in Dashtiary cattle, respectively. The both of population demonstrated deviation from Hardy-Weinberg equilibrium in FABP4 (P<0.05). Heterozygosity index includes Shanen index (I), Nei index, observed and expected heterozygosity investigated 0.48, 0.30, 0.3 and 0.30 in Sistani population and 0.39, 0.11, 0.27 and 0.24 in Dashtiary population, respectively. The association between the patterns of genotypes and growth-related traits explained a significant effect with body weight in 6, 9 and 12 months, but it was not significant effect on birth weight and weaning weight.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | FABP4 gene, Sistani and Dashtieary cattle, PCR-RFLP, Growth related traits |
| Subjects: | S Agriculture > SF Animal culture |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 29 May 2017 07:31 |
| Last Modified: | 29 May 2017 07:31 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/1396 |
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