Flavonol synthase (FLS) gene expression using manganez and jasmonic acid in plant (Capparis spinosa)

Azhdarpour, Nafiseh (2016) Flavonol synthase (FLS) gene expression using manganez and jasmonic acid in plant (Capparis spinosa). Masters thesis, university of zabol.

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Abstract

Blind family with the scientific name capparis spinosa beony to cobris family with ingre dients flavonoid, saponins, essence, pectin, resins, glycoside. One of flavonoids in spinosa, is quercetin. Quercetin during a biosynthetic pathway(phenyl propanoid) quercetin hydroxy freedom can be ob tained from flavonol synthase enzymes(FLS). In this study the expression of flavonol synthase under the in fluence of manganese and acid jasmonic in spinosa asa factorial experiment in a complete block desing with 3 replication are studied. In order for the germination of seeds of blind patients using chilling and heating, seeds blind and plastic pots ofter the emergence of the seedling section consists of 2stage air plant in seedling before flowering by manganese concentration of 80ppm and jasmonic acid was reduced to 50micro from 48 and 72 hours was harvest. After harvesting the leaves to extract RNA extraction kit according to the instruetions RNA(Dena EPA) was used proposed the manufacturer.corbett Rotor Gene 3000 were using for real time PCRte chnigues.the results are basedon melting curve analysis and charts represent different stage of amplification usingwas performed(ΔΔct)and results shawed that the presence of manganese and jasmonic acid in the production of reactive oxygen species sothat plants with in creased expression of other genes in the biosynthesis of flavonoids and increasing flavonoid content is to reduce the resulting tensions according to spinosa treatments on the treatment applied jasmonic an says that the treatment of manganese in the 48 hours after applying better respond to the increased expression has been shown and treatment in teraction effect of jasmonic+manganese appropriate increase compared to control hasshown, on the other hand 72 hours of the treatment period had a significant reducation.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Capparis spinosa, Gene Expression, Flavonol Synthase, Quercetine
Subjects: S Agriculture > SB Plant culture
Depositing User: admin admin1 admin2
Date Deposited: 13 May 2017 04:14
Last Modified: 13 May 2017 04:14
URI: http://eprints.uoz.ac.ir/id/eprint/1324

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