Evaluation of TSLP and NF-κB Genes Expression In Gastric Cancer Patients

Rahimi, Elham (2016) Evaluation of TSLP and NF-κB Genes Expression In Gastric Cancer Patients. Masters thesis, University of Zabol.

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NF-κB is a transcription factor that plays important role in regulating immune and inflammatory responses. The purpose of this study was to determine whether NF-κB is constitutively activated in human gastric carcinoma tissues, its correlation with TSLP, As well as the relationship both of two protein with H. pylori and to determine any correlation between RelA activity with clinicopathological features of gastric cancer (GC). In this study, fresh tumoral tissues and distant tumor-free samples from 48 GC patients were assessed for RelA mRNA expression by quantitative real-time PCR. The GC samples were also assessed for H. pylori DNA using primers specific for H.pylori 16S rRNA and the UreC genes by PCR. TSLP Expressionin were evaluated by quantitative real-time PCR, was performed. RelA mRNA was overexpressed in 25 of the 48 (52.1 %) GC samples relative to their corresponding normal tissues. Rel A overexpression was significantly correlated with TSLP overexpression. Of the 25 patients with RelA overexpression, 20 (75.0 %) had TSLP overexpression (p = 0.000). Other variables havent a significant relationship with Rel A, also A significant correlation between co-overexpression of TSLP and RelA genes with other variants and H.pylori was not found. Because in this study dispersion of RelA was very low, only one sample was under-expressand all were Over-expressand or No change.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Gastric cancer- NF-kB p65 (RelA) - TSLP - H. pylori - Real-time PCR
Subjects: Q Science > QH Natural history > QH426 Genetics
Depositing User: admin admin1 admin2
Date Deposited: 09 May 2017 03:30
Last Modified: 09 May 2017 03:30
URI: http://eprints.uoz.ac.ir/id/eprint/1314

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