Study of MYB gene expression in 5 sistan bread wheat cultivars under drought stress

Tabaraki, Hadis (2016) Study of MYB gene expression in 5 sistan bread wheat cultivars under drought stress. Masters thesis, University of Zabol.

Study of MYB gene expression in 5 sistan bread wheat.pdf

Download (348kB) | Preview


Drought is one of the most important constraining environmental stresses for agricultural products that is dominated in significant areas of Iranian farmlands (agricultural lands). Drought tolerance morphological recognition plays an important role in improving problems caused under drought conditions. In fact, the genetic relationship of these processes and their precise relationship with the efficiency level of plant seed are important and unknown. Therefore, in order to investigate morphological characteristics Physiological attributes measured included: photosynthetic pigments (chlorophylls a, b, carotenoid and total), antioxidant enzymes (catalase, ascorbate peroxidase, guaiacol peroxidase, and poly‐phenol oxidase) and expression of TaMYB73gene under drought stress two studies were performed using three varieties of bread wheat (Chamran2, Afghani calk, Sistan, Arg and Ofogh). In order to investigate morphological characteristics related to drought tolerance, in the first study five varieties of bread wheat, including Chamran2, Afghani calk, Sistan, Arg and Ofogh were evaluated using a Factorial test and in a completely randomized design with five replicates. Drought had a significant effect on some study traits (stem length, leaf length, leaf width, shoot fresh weight, shoot dry weight, rootlet number, . root number) and interaction of drought. Cultivar was significant for rootlet dry weight and stem length. Thus, varieties showed different reaction in different irrigation regims for characteristics. The second test was performed in order to investigate the expression pattern of TaMYB73 gene which is of the effective regulatory genes in response to stresses. Sampling was performed randomly for leaves in each pot. Leaves were frozen in liquid nitrogen, then finally kept in freezer at -80 °C. After RNA extraction and synthesis of cDNA, the expression of TaMYB73gene, which is a gene related to transcription factor, was compared and evaluated using Real-time PCR in three varieties of wheat under drought conditions. The 18s house keeping gene was used to normalize the data. The results revealed an increase in gene expression in TaMYB73 under drought stress and the effect of stress and species in both genes in the 1 level percent displayed a meaningful difference, which in turn shows the impact of drought stress on these genes expressions. In this study, Chamran2 and Arg species, especially Chamran2, in regards with all the examined attributes, in drought conditions compared to the base line returned the most favorable results, identifying them as drought tolerant species.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Triticum aestivum, Real-time PCR, Physiological characteristics, MYB gene
Subjects: S Agriculture > SB Plant culture
Depositing User: admin admin1 admin2
Date Deposited: 19 Apr 2017 06:42
Last Modified: 19 Apr 2017 06:42

Actions (login required)

View Item View Item