Jalilian, S. (2016) The effect of colchicine on the pathways of apoptosis in prostate cancer cell lines pc3. Masters thesis, University of Zabol.
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Abstract
Colchicine is an alkaloid that has been widely used to treat gout. This alkaloid exerts anti-proliferative effects on malignant tumors and inhibits their metastasis. Colchicine activates intrinsic pathway of apoptosis to induce cell death. Bax and Bcl2 genes are among the key target genes of internal pathway; thus the present study aims to determine the effect of colchicine on the expression of these two genes on pc3 cell line (prostate cancer). After the cell line was properly cultured, cells were treated with different concentration of colchicine and the proliferative inhibitory effect of this molecule was assayed using MTT method. Afterwards, the RNA content of cells was extracted using an RNA extraction kit. Then the cDNA was synthesized for Bax and Bcl2 genes and finally Real time PCR was run. MTT results approved the inhibitory effect of colchicine wild the results from Real time PCR cleared out a drastic increase in the expression of pro-apoptotic gene of Bax in comparison to controls not treated with colchicine. On the other hand, the expression of anti-apoptotic gene of Bcl2 was significantly decreased compared to control. Altogether, it could be concluded that colchicine is able to induce apoptosis by affecting genes involved in this pathway such as Bax and Bcl2 therefore inhibit cancer cell growth and metastasis.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | colchicine, PC3, Apoptosis, Bcl-2, Bax |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 20 Dec 2016 07:01 |
| Last Modified: | 20 Dec 2016 07:01 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/1174 |
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