Sobhanizadeh, Ali (2016) Optimization of callus induction investigating the effect of biotic and abiotic elicitors on amount of phenols compound of medicinal plant Nigella sativa in vitro. Masters thesis, University of Zabol.
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Abstract
Nigella (Nigella sativa) is a plant from Buttercup family and one of the country's most important medicinal plants. This study aimed to optimization of callus formation and the investigation of the effect of two Elicitors of yeast extract and Nano silver on the amount of phenol and flavonoid of Black Cumin conducted under laboratory conditions. The treatments of this study includes callus induction, regeneration (direct), and the application of elicitors (twice) which were done based on completely randomized design with three replications. The studied traits in callus induction test included callus formation percentage, size, color, appearance, fresh and dry weight of callus, and in the analyzes of the implication of elicitor phenolic changes rates was measured through liquid phase chromatography. The results showed that the stem explants with 25/0 BAP, 4 2,4D mg / l on callus induction and cotyledon explants with 5/0 BAP, 2 2,4D mg / l had the greatest impact on the volume of Callus. Also in terms of fresh and dry weight Cotyledon was the best explant. In the explant regeneration, 1 24D ، 5/0 BAP mg/l and root explants was the best treatment. The results of elicitor represent the effect of yeast extract in 250 ppm in the range of 7 days and Nano silver treatment ppm 60 with yeast extract250 ppm in the range of 3 days had the highest role in the raise of flavonoid. Likewise, the results of HPLC showed that greatest impact of Nano-silver 30 ppm treatment in the range of7 days and the yeast extract 500 ppm in the range of 3 days on secondary metabolites of Quercetin.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Nigella Sativa, tissue culture, elicitor, secondary metabolism |
| Subjects: | S Agriculture > SB Plant culture |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 19 Dec 2016 10:01 |
| Last Modified: | 19 Dec 2016 10:01 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/1144 |
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