Investigation of the expression level of iss and bor genes in Avain Pathogenic Escherichia coli inculated in chicken serum

Abasi, Sommaye (2016) Investigation of the expression level of iss and bor genes in Avain Pathogenic Escherichia coli inculated in chicken serum. Masters thesis, University of Zabol.

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Colibacilosis is one of the most important bacterial diseases in poultries which is frequently observed in Iran. Data about characteristics of acuity factors of E. coli, isolated from colibasilosis lesions is very limited. Identification of E. coli acuity factors in experimental studies and the correlation between acuity and resistance to serum, have increased people insight about disease procedure in infections caused by this bacterium and helped significantly identifying pathogenic strains and control of E. coli in poultry industry. There is low information about genes contributed in acuity of APEC in Zabol region. Obviously, a comprehensive analysis of mechanisms of serum resistance, need detailed studies about bacterium genes which are responsible for serum resistance. Based on available references, iss and bor genes are involved in developing serum complement resistance in pathogenic E. coli of poultries. With regards to this fact and also 90% similarity between amino acid sequences of iss and bor genes, simultaneous study of these two genes can be helpful. In the present study, the rate of iss and bor genes expression and the effect of time in expression rate of these two genes in pathogenic E. coli of poultries in Zabol and the resistance created in the bacterium by these two genes encountering serum were evaluated. After evaluation of serum efficacy, and confirmation of presence of iss, bor and gapdh genes in pathogenic E. coli of poultries, exposition of bacterium with serum was performed and 24, 28 and 32 hours of incubation was done. After that, the expression of iss, bor and graph genes in isolated E. coli from poultries were performed by semi quantitative RT-PCR. The results showed that, the expression level of bor gene in exposition with serum was significantly higher than expression of iss gene. Whiles, the expression of iss gene was significantly increased by time, but the expression rate of bor was decreased. With regards to these results, factors such as difference in number of duplication of genes, difference in location of genes in each strain, the effect of alteration processes and limitation in exposition with serum, other usages of bor gene and presence of other genes involved in serum resistance in higher expression of bor compared to iss gene, play roles.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Keywords: pathogenic E. coli of poultries, multiplier gene of serum resistance, serum resistance, Semi quantitative reverse transcriptase polymerase chain reaction, bor
Subjects: S Agriculture > SF Animal culture
Depositing User: admin admin1 admin2
Date Deposited: 19 Dec 2016 07:28
Last Modified: 19 Dec 2016 07:28

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