Prevalence of Pathogenicity island markers in uropathogenic Escherichia coli isolated from patients attending Zabol teaching hospitals

Sholibor, M. (2016) Prevalence of Pathogenicity island markers in uropathogenic Escherichia coli isolated from patients attending Zabol teaching hospitals. Masters thesis, University of Zabol.

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Uropathogenic Escherichia coli is the most common cause of urinary tract infection (UTI). The ability of E. coli to cause UTI is associated with specific virulence determinants, some of which are encoded on pathogenicity islands markers (PAI). The first step is to confront and inhibit of this pathogen is the identification genetic traits associated with the pathogenicity of pathogenicity islands markers, virulence genes. The main objective of this study was to determine the frequency of pathogenicity islands include PAI of I536, II536, ICFT073, IJ96, IIJ96 which encoded the α- hemolysin and PAI of IJ96, ICFT073, IICFT073 that are fimbriae encoder, virulence genes include: traT Encoding outer membrane, lipoprotein Iron acquisition systems gene fyuA,and Aerobactin receptor iutA, Serum resistance genes (K1 ، traT, kpsII )and gene encoding colicin V which called cvaC gene and also the type of phylogeny groups was determined using Multiplex PCR method. A total of the 100 Escherichia coli isolates, pathogenicity islands PAI IV536 with 84% highest prevalence and pathogenicity islands PAI IJ96, PAI I536 do not exist, respectively. Frequency pathogenicity islands for islands PAI IIJ96, PAI IICFT073, PAI ICFT073, PAI II536 were detected 9%, 30%, 44%, 16% respectively. Gene frequency for fyuA ،iutA, K1, kpsII, genes were detected 79%, 76%, 15%and 68%, respectively. About 55, 22, 17 and 6 isolates belonged to B2, D, A and B1 phylogeny groups, respectively. The highest of the virulence gene distribution was observed in group B2 and the lowest in groups B1 and A. The most significant association between the phylogeny groups and pathogenicity islands and virulence genes was observed in isolates belonging to groups A and B2, respectively. Group B1 isolates had no significant correlation with the distribution of any genes (P value ≤ 0.05). Generally results of this study showed that Pathogenicity island PAI IV536 and genes fyuA ،kpsII, iutA, traT are more abundant and important in isolates of Sistan region.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Uropathogenic Escherichia coli, Urinary tract infection, Pathogenicity island markers, Virulence genes, Phylogeny groups
Subjects: Q Science > QH Natural history > QH301 Biology
Depositing User: admin admin1 admin2
Date Deposited: 04 Dec 2016 06:42
Last Modified: 04 Dec 2016 06:42

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