Shahraki, M. (2014) Prevalence of CTX-M‚ TEM‚ SHV genes in extended-spectrum betalactamase- producing Enterobacters isolated from patients attending in zabol hospitals using PCR method. Masters thesis, University of Zabol.
|
Text
Prevalence of CTX-M‚ TEM‚ SHV genes in extended-spectrum beta-lactamase-producing.pdf Download (286kB) | Preview |
Abstract
Extended-spectrum beta-lactamase of CTX-M٫TEM and SHV types is considered as an important mechanism resistant to cephalosporin in gram-negative patogene and is widely growing. Enterobacter species are able to produce extended-spectrum beta-lactamase (ESBLs).The aim of this study was to detect the prevalence of CTX-M٫TEM and SHV genes in ESBLs producing entrobacters isolated from patients attending in zabol hospitals using PCR method. In this analytical-descriptive study٫ antibacterial susceptibility patterns of 165 enterobacter to Cefotaxim٫ Ceftazidim٫ Ceftriaxone and Azteronam tested using disk diffusion method. In addition٫ confirmatory tests for detecting ESBLs phenotypes were performed using Ceftazidim-clavulanic acid combination disk. The presence of CTX-M٫ TEM and SHV genes were assessed using PCR. Confirmatory phenotypic test showed 92٪ of the strains were ESBL positive. The prevalence of CTX-M٫ TEM and SHV genes in isolated Enterobacters was 63٪ ٫ 72٪ and 76 ٫ ٪ respectively. High frequency of CTX-M٫TEM and SHV genes in ESBL producing isolates indicates that this enzymes plays an important role in resistance to betalactam containing antibiotics.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | CTX-M gens٫ TEM gens٫ SHV gens٫ Enterobacter٫ Extended-spectrum beta-lactamases٫ Polymerase Chain Reaction |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Depositing User: | admin admin1 admin2 |
| Date Deposited: | 04 Dec 2016 06:35 |
| Last Modified: | 04 Dec 2016 06:35 |
| URI: | http://eprints.uoz.ac.ir/id/eprint/1064 |
Actions (login required)
 |
View Item |
