Mapping genomic regions of physiological and Morphological traits of barley under nickel Stress conditions under hydroponic culture

Golshani, Farshid (2014) Mapping genomic regions of physiological and Morphological traits of barley under nickel Stress conditions under hydroponic culture. Masters thesis, University of Zabol.

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In order to map genomic regions related nickel stress in barley and determine the contribution of each QTL on trait variation, 72 doubled haploid lines derived from crosses of with two parents were studied Steptoe and Morex. This research derived from Agricultural Biotechnology Research Institute of Zabol University in the hydroponic environment in 2013. The experiments were arranged as to completely randomized block design, with two replicates and two treatments non stress and stress nickel. The studied traits morphologic traits and physiologic traits including: As root length, coleoptiles, stem, leaf, total, the ratio of root to shoot and Fresh weight of root, shoot, total and weight ratio of root to shoot, root dry weight, Chlorophyll ratio, Relative water content, Membrane stability index and Chlorophyll fluorescence And rate nickel, zinc, copper, manganese and iron in the root, shoot, total and ratio. The statistical analysis for phenotypic traits Reviews Analysis of variance, combined analysis calculated phenotypic correlations between traits, factor analysis and cluster analysis was performed. Analysis of variance result showed significant difference among the lines in two stress and non stress nickel for most of the traits. The maximum correlation in non stress conditions were observed between Ni root with total Ni (r= 0.944**) and total Mn with Mn shoot (r= 0.947**). Maximum correlation In non stress condition between traits between Ni root with total Ni (r= 0.984**) and total Ni with Ni shoot (r= 0.989**). In The factor analysis, the first 13 factor 85/83% of the total variability in non stress condition and the first 12 factor 84/38% of the total variability in stress condition explained. Factor analysis extracted characters taken into consideration and named as Ni factor, Cu, Zn, length, weight, morphological and photosynthetic capacity. Using cluster analysis grouped genotypes non stress and Ni stress conditions 6, for each, were identified. QTL analysis was carried out using genetic linkage map derived from 327 marker of RFLP and QTL cartographer software with composite interval mapping method. In total 114 QTL was found for the traits (61 QTL for non stress condition and 53 QTL for stress condition). Phenotypic variation that were explained by these QTLs, varied from 10.09-54.82%. The highest phenotypic variances were related to total Mn in non stress (QMnk7H.3n) conditions and lowest phenotypic variances were related to The amount of total Mn in non stress (QMnk2H.1n) conditions. LOD ranged between 2.5-5.41. The highest and lowest LOD were attained for the QTLs of Root fresh weight (Qwaz2H.1s) and Root to shoot Ni (QNi-r/s4H.1s) ratio non stress condition. In this study, a total of five QTL position stable qualified traits Coleoptiles length, total nickel, Zn root and total iron obtained. Therefore, if this result would repeat in different environment, year and genotypes, it can be used in marker assisted selection

Item Type: Thesis (Masters)
Uncontrolled Keywords: Barley, Doubled haploid lines, Hydroponic, Nickel stress, QTL
Subjects: S Agriculture > SB Plant culture
Depositing User: admin admin1 admin2
Date Deposited: 23 Feb 2016 05:53
Last Modified: 23 Feb 2016 05:53

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